The region of the marine environment between shallow waters and deep-sea (30-150 m in depth) is referred to as the twilight or mesophotic zone. This zone has been scarcely examined, as these depths are too shallow for safe submersible operation. Since 2018, a survey of mesophotic echinoderms in Korea has yielded many specimens of interest. In this study, we present two newly recorded echinoderms, Henricia irregularis and Parastichopus nigripunctatus based on morphological redescriptions with high-definition photographs and DNA barcoding data for P. nigripunctatus.