In this study gobiid fish species were investigated at 31 stations from the northeastern Aegean Sea. Different habitat types between 0 and 50 m depth were studied by scuba diving with newly modified underwater visual census with ranked prevalence using time-transect. Thirty-three species were determined as belonging to family Gobiidae. Distribution, abundance, and some ecological properties of the species were examined. Considering this, four of the observed species determined as very common, seven as common, ten as locally common, six as rare, and six as very rare in the area. Pseudaphya ferreri, Gammogobius steinitzi, and Corcyrogobius liechtensteini were determined for the first time for the area. Detailed morphological characters of some species are also given.